In step 3 of ß-oxidation, which molecule is produced from L-ß-hydroxy-acyl-CoA?

In step 3 of ß-oxidation, L-β-hydroxy-acyl-CoA undergoes a reaction in which it is oxidized to form β-ketoacyl-CoA. This step involves the enzyme L-β-hydroxyacyl-CoA dehydrogenase, which facilitates the conversion by removing two hydrogen atoms from the substrate, leading to the formation of a double bond and the creation of a keto group at the β-position. The production of β-ketoacyl-CoA is crucial for the continuity of the β-oxidation cycle, as this intermediate is then acted upon in the subsequent step, which involves cleavage and the release of another molecule of fatty acyl-CoA or acetyl-CoA, depending on the chain length of the fatty acid. Thus, the correct answer emphasizes the significance of recognizing the transformation occurring during this particular phase of fatty acid metabolism.